Sclerostin domain-containing protein 1 (Sostdc1), rat, recombinant

Organism: Rattus norvegicus (Rat). Source: Yeast. Expression Region: 24-206aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-sumostar-tagged. Target Protein Sequence: FKNDATEILY SHVVKPVSAH PSSNSTLNQA RNGGRHFSST GLDRNSRVQV GCRELRSTKY ISDGQCTSIS PLKELVCAGE CLPLPVLPNW IGGGYGTKYW SRRSSQEWRC VNDKTRTQRI QLQCQDGSTR TYKITVVTAC KCKRYTRQHN ESSHNFESVS PAKPAQHHRE RKRASKSSKH SLS. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Directly antagonizes activity of BMP2, BMP4, BMP6 and BMP7 in a dose-dependent manner. May be involved in the onset of endometrial receptivity for implantation/sensitization for the decidual cell reaction. Enhances Wnt signaling and inhibits TGF-beta signaling. Reference: "The WNT signalling modulator, Wise, is expressed in an interaction-dependent manner during hair-follicle cycling." O'Shaughnessy R.F.L., Yeo W., Gautier J., Jahoda C.A.B., Christiano A.M. J. Invest. Dermatol. 123:613-621(2004). Function: Directly antagonizes activity of BMP2, BMP4, BMP6 and BMP7 in a dose-dependent manner (By similarity). May be involved in the onset of endometrial receptivity for implantation/sensitization for the decidual cell reaction. Enhances Wnt signaling and inhibits TGF-beta signaling.