Protein Wnt-9a (Wnt9a), recombinant mouse

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 30-365aa. Protein Length: Full Length of Mature Protein. Tag Info: C-terminal 6xHis-tagged. Target Protein Sequence: YFGLTGSEPL TILPLTLETE AAAQAHYKAC DRLKLERKQR RMCRRDPGVA ETLVEAVSMS ALECQYQFRF ERWNCTLEGR YRASLLKRGF KETAFLYAIS SAGLTHALAK ACSAGRMERC TCDEAPDLEN REAWQWGGCG DNLKYSSKFV KEFLGRRSSK DLRARVDFHN NLVGVKVIKA GVETTCKCHG VSGSCTVRTC WRQLAPFHEV GKHLKHKYET SLKVGSTTNE ATGEAGAISP PRGRASGSGG GDPLPRTPEL VHLDDSPSFC LAGRFSPGTA GRRCHREKNC ESICCGRGHN TQSRVVTRPC QCQVRWCCYV ECRQCTQREE VYTCKG. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: Not Test. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. Relevance: Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt/beta-catenin signaling pathway (By similarity). Required for normal timing of IHH expression during embryonic bone development, normal chondrocyte maturation and for normal bone mineralization during embryonic bone development. Plays a redundant role in maintaining joint integrity . Reference: n/a. Function: nan