Pleiotrophin, active (PTN), Recombinant, Human

Secreted growth factor that induces neurite outgrowth and which is mitogenic for fibroblasts, epithelial, and endothelial cells. Binds anaplastic lymphoma kinase (ALK) which induces MAPK pathway activation, an important step in the anti-apoptotic signaling of PTN and regulation of cell proliferation. {ECO:0000269, PubMed:11278720, ECO:0000269, PubMed:1768439}. Biological Activity: Fully biologically active when compared to standard. The biological activity was measured by its ability to enhance neurite outgrowth of E16­E18 rat embryonic cortical neurons, when neurons were plated on 96 well culture plates that had been pre­coated with 100 ul/well of a solution of 5­10 ug/ml rHuPTN. Sequence: GKKEKPEKKV KKSDCGEWQW SVCVPTSGDC GLGTREGTRT GAECKQTMKT QRCKIPCNWK KQFGAECKYQ FQAWGECDLN TALKTRTGSL KRALHNAECQ KTVTISKPCG KLTKPKPQAE SKKKKKEGKK QEKMLD, Molecular Weight: 15.3kD, PubMed ID: 1701634, 2270483, 1768439, 1457401, 8484754, 9299545, 14702039, 12690205, 15489334, 2388713, 1733956, 8241100, 8484780, 11278720, 20873783, Gene Name: PTN,HBNF1,NEGF1, Swiss Prot: P21246, Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.