OSM, Recombinant, Human (Oncostatin-M, Oncostatin M)

Oncostatin M (OSM) is a growth and differentiation factor that participates in the regulation of neurogenesis, osteogenesis and hematopoiesis. Produced by activate T cells, monocytes and Kaposi's sarcoma cells, OSH can exert both stimulatory and inhibitory effects on cell proliferation. It stimulates the proliferation of fibroblasts, smooth muscle cells and Kaposi's sarcoma cells, but, inhibits the growth of some normal and tumor cell lines. It also promotes cytokine release (e.g. IL-6, GM-CSF and G-CSF) from endothelial cells, and enhances the expression of low-density lipoprotein receptor in hepatoma cells. OSM share several structural and functional characteristics with LIF, IL-6, and CNTF. Human OSM is active on murine cells. The human OSM gene encodes for a 252aa polypeptide, containing 25aa signal sequence for secretion and a 227 precursor protein. Proteolytic processing of this precursor removes an 18aa C-terminal peptide and generates the mature OSM form. Recombinant human Oncostatin M is a 23.9kD protein, containing 209aa. Source: Recombinant protein corresponding to human Oncostatin M, expressed in E.coli. Biological Activity: Determined by the dose-dependent stimulation of the proliferation of human TF-1 cells. The ED50 was found to be 2ng/ml, Specific Activity: 5x10e5units/mg, Endotoxin: <0.1ng/ug (1EU/ug), AA Sequence: MAAIGSCSKE YRVLLGQLQK QTDLMQDTSR LLDPYIRIQG LDVPKLREHC RERPGAFPSE ETLRGLGRRG FLQTLNATLG CVLHRLADLE QRLPKAQDLE RSGLNIEDLE KLQMARPNIL GLRNNIYCMA QLLDNSDTAE PTKAGRGASQ PPTPTPASDA FQRKLEGCRF LHGYHRFMHS VGRVFSKWGE SPNRSRRHSP HQALRKGVRR, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.