Oncostatin M (209 a.a.) (human), animal free recombinant

Oncostatin M (OSM) is a multifunctional growth and differentiation factor involved in regulating neurogenesis, osteogenesis, and hematopoiesis. It is produced by activated T cells, monocytes, and Kaposi's sarcoma cells, exerting both stimulatory and inhibitory effects on cell proliferation. OSM stimulates the growth of fibroblasts, smooth muscle cells, and Kaposi's sarcoma cells, while it inhibits the proliferation of certain normal and tumor cell lines. Additionally, OSM promotes the release of cytokines like IL-6, GM-CSF, and G-CSF from endothelial cells and enhances low-density lipoprotein receptor expression in hepatoma cells. Structurally and functionally, OSM shares characteristics with LIF, IL-6, and CNTF. It is biologically active on murine cells. The human OSM gene encodes a 252 amino acid polypeptide with a 25 amino acid signal sequence for secretion, forming a 227 amino acid precursor protein. Proteolytic cleavage of this precursor removes an 18 amino acid C-terminal peptide to yield the mature OSM form. Recombinant Human Oncostatin M is a 23.6 kDa protein consisting of 209 amino acid residues. Product Specifications Species Human Published Species Human Expression System E.Coli Amino Acid Sequence AAIGSCSKEY RVLLGQLQKQ TDLMQDTSRL LDPYIRIQGL DVPKLREHCR ERPGAFPSEE TLRGLGRRGF LQTLNATLGC VLHRLADLEQ RLPKAQDLER SGLNIEDLEK LQMARPNILG LRNNIYCMAQ LLDNSDTAEP TKAGRGASQP PTPTPASDAF QRKLEGCRFL HGYHRFMHSV GRVFSKWGES PNRSRRHSPH QALRKGVRR Molecular Weight 23.6 kDa Class Recombinant Type Protein Purity > 98% by SDS-PAGE gel and HPLC analyses Endotoxin concentration <0.1 EU/µg Activity Determined by its ability to stimulate the proliferation of human TF-1 cells. The expected ED50 is = 2.0 ng/ml, corresponding to a specific activity of > 5 x 105 units/mg. Form Lyophilized Contains No Preservative CAUTION For Research Use Only. Not for use in diagnostic procedures.Protein function: Growth regulator. Inhibits the proliferation of a number of tumor cell lines. Stimulates proliferation of AIDS-KS cells. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. Uses both type I OSM receptor (heterodimers composed of LIFR and IL6ST) and type II OSM receptor (heterodimers composed of OSMR and IL6ST). Involved in the maturation of fetal hepatocytes, thereby promoting liver development and regeneration. [The UniProt Consortium]