Midkine (RIHB), chicken, recombinant

Organism: Gallus gallus (Chicken). Source: E.coli. Expression Region: 22-142aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: AKAKKEKMKK EGSECQDWHW GPCIPNSKDC GLGYREGSCG DESRKLKCKI PCNWKKKFGA DCKYKFESWG GCSAKTGVKT RSGILKKALY NAECEEVVYV SKPCTAKMKA KAKAKKGKGK D. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Has mitogenic activity, and neurite extension activity for PC12 cells. Reference: "Identification of a new heparin-binding protein localized within chick basement membranes." Vigny M., Raulais D., Puzenat N., Duprez D., Hartman M.P., Jeanny J.C., Courtois Y. Eur. J. Biochem. 186:733-740(1989). Function: nan