Melanoma-derived growth regulatory, active (MIA), Recombinant, Human

Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas. Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human A375 cell line is less than 5ug/ml, corresponding to a specific activity of ? 200 IU/mg. Sequence: GPMPKLADRK LCADQECSHP ISMAVALQDY MAPDCRFLTI HRGQVVYVFS KLKGRGRLFW GGSVQGDYYG DLAARLGYFP SSIVREDQTL KPGKVDVKTD KWDFYCQ, Molecular Weight: 12.1kD, PubMed ID: 7923218, 8550608, 12230496, 15489334, 15340161, 19807924, 22419821, 11331761, Gene Name: MIA, Swiss Prot: Q16674, Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.