M-CSF, (CSF-1), rHuman,Animal Free,High Purity/Bioactive

M-CSF (macrophage colony stimulating factor, colony stimulating factor 1, CSF-1) is a hemtopoietic growth factor produced by monocytes, granulocytes, endothelial cells, fibroblasts and activated B-cells and T-cells. M-CSF stimulates macrophage colony formation and regulates proliferation and differentiation of hematopoeitic stem cells into macrophages and regulates survival and differentiation of monocytes. It enhances antibody-dependent cell-mediated cytotoxicity, enhances marophage killing of tumor cells and microorganisms, regulates the release of cytokines and inhibits bone resorption by osteoclasts. Human M-CSF shows biological activity on murine and rat cells and although murine M-CSF is active in rat cells it is inactive in human cells. Source: Produced in the endosperm tissue of barley grain (Hordeum vulgare). Formulation: PBS pH 7.2, sterile filtered through 0.2 µm filter. Purity: Greater than 95% by SDS-PAGE gel analysis. Reconstitution: Note: Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile buffered saline to a concentration of no less than 100 µg/ml. For long term storage of the reconstituted solution it is recommended to add a carrier protein (0.1% HSA or BSA). Stability: The lyophilized protein, though stable at room temperature for few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles. Biological Activity: The bioactivity of ISOkine(R) recombinant human M-CSF was assayed by measuring its dose dependent effect on proliferation of M-NFS-60 cells. The ED50 value for this effect is < 4 ng/ml corresponding to specific activity > 2.5 x 10e5 U/mg. We recommend that the optimal concentration for each specific application be determined by an initial dose-response assay. Each batch of ISOkine(R) growth factor is tested for bioactivity and verified to have comparable activity to a commercial source. Endotoxin Level: Endotoxin level is less than 0.005ng per µg of ISOkine(R) product (0.05EU/µg). MAT Assay: Purified ISOkine(R) product carries no pyrogenic or pro-inflammatory contaminants, as assayed with monocyte activation test using Human 10-plex Cytokine Assay measuring IL-6, TNF-alpha and IL-1 beta induction. ** Ref. The Blood Bank, University Hospital of Iceland, Reykjavik, Iceland Molecular Weight: Recombinant human M-CSF contains 159 amino acids and a 16 a.a. Histidine-based tag for a total length of 175a.a. and has a predicted molecular mass of 20.7 kDa. The recombinant protein migrates with an apparent molecular mass of 24-26 kDa in SDS-PAGE.