Leukocyte cell-derived chemotaxin-2 (Lect2), mouse, recombinant

Organism: Mus musculus (Mouse). Source: Yeast. Expression Region: 19-151aa. Protein Length: Full Length of Mature Protein. Tag Info: C-terminal 6xHis-Myc-tagged. Target Protein Sequence: GPWANICASK SSNEIRTCDS YGCGQYSAQR TQRHHPGVDV LCSDGSVVYA PFTGKIVGQE KPYRNKNAIN DGIRLSGRGF CVKIFYIKPI KYKGSIKKGE KLGTLLPLQK IYPGIQSHVH VENCDSSDPT AYL. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Has a neutrophil chemotactic activity. Also a positive regulator of chondrocyte proliferation. Reference: "Molecular cloning of mouse and bovine chondromodulin-II cDNAs and the growth-promoting actions of bovine recombinant protein." Shukunami C., Kondo J., Wakai H., Takahashi K., Inoue H., Kamizono A., Hiraki Y. J. Biochem. 125:436-442(1999). Function: nan