Latent-transforming growth factor beta-binding protein 4 (Ltbp4), partial, mouse, recombinant

Organism: Mus musculus (Mouse). Source: Yeast. Expression Region: 1223-1666aa. Protein Length: Partial. Tag Info: C-terminal 6xHis-tagged. Target Protein Sequence: RECYFDTAAP DACDNILARN VTWQECCCTV GEGWGSGCRI QQCPGTETAE YQSLCPHGRG YLVPSGDLSA RRDVDECQLF QDQVCKSGVC VNTAPGYSCY CSNGFYYHAH RLECVDNDEC ADEEPACEGG RCVNTVGSYH CTCEPPLVLD GSRRRCVSNE SQSLDDNLGV CWQEVGPDLV CSRPRLDRQA TYTECCCLYG EAWGMDCALC PAQDSDDFEA LCNVLRPPAY GPPRPGGFGI PYEYGPDIGP PYQSLPYGPD LYPPPVLPYD PYPPPPGPFA RREAPYGAPP FDMPDFEDDG GPYGESETPD PPSRGTGWPY RSRDTRGSFP EPEESSERGS YTGALSEPYE GLEAEECGIL DGCPHGRCVR VPEGFTCDCF DGYRLDITRM SCVDVNECDE AEATSPLCVN ARCVNTDGSF RCICRPGFAP THQPHHCAPA RPRA. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Key regulator of transforming growth factor beta (TGFB1, TGFB2 and TGFB3) that controls TGF-beta activation by maintaining it in a latent state during storage in extracellular space. Associates specifically via disulfide bonds with the Latency-associated peptide (LAP), which is the regulatory chain of TGF-beta, and regulates integrin-dependent activation of TGF-beta. Reference: "Modeling autosomal recessive cutis laxa type 1C in mice reveals distinct functions for Ltbp-4 isoforms." Bultmann-Mellin I., Conradi A., Maul A.C., Dinger K., Wempe F., Wohl A.P., Imhof T., Wunderlich F.T., Bunck A.C., Nakamura T., Koli K., Bloch W., Ghanem A., Heinz A., von Melchner H., Sengle G., Sterner-Kock A. Dis. Model. Mech. 8:403-415(2015). Function: nan