Interleukin 10R, beta, Fc Chimera, Recombinant, Mouse, aa21-220 (IL-10 R beta, CDw210b, CRFB4, D21S5

Interleukin­10 Receptor beta (IL­10 R beta), also known as IL­10 R2 and CRF2­4, is a 60kD transmembrane glycoprotein that functions as a co­receptor for several class 2 cytokines including Interleukins­10, ­22, ­26, ­28A/IFN­gamma2, ­28B/IFN­gamma3, and ­29/IFN­gamma (1, 2). IL­10 R beta associates with ligand­specific receptor subunits to form signaling receptor complexes, e.g. IL­10 R alpha for IL­10 (3, 4), IL­20 R alpha for IL­26 (5, 6), IL­22 R alpha for IL­22 (7, 8), and IL­28 R alpha for IL­28A, IL­28B, and IL­29 (9, 10). IL­10 R beta is widely expressed, while the associated receptor subunits exhibit differential expression patterns (1). The ligand­specific subunits are responsible for the divergent functions of these cytokines, encompassing immune suppression, promotion or inhibition of inflammation, mucosal defense, antiviral immunity, and hematopoiesis (1). IL­10 R beta deficient mice lack responsiveness to each of those cytokines. IL­10 R beta contributes to ligand binding, but effective signaling is only triggered in the presence of the ligand­specific subunit (8, 9, 11). In the case of IL­10, a cytokine dimer binds to two IL­10 R alpha/IL­10R1 chains, resulting in recruitment of two IL­10 R beta/IL­10R2 chains (3, 12). Some members of the IL­10 family are monomeric cytokines and interact with single molecules of IL­10 R beta and their ligand­specific subunit (1). Mature mouse IL­10 R beta consists of a 201 amino acid (aa) extracellular region with two fibronectin type­III domains, a 21 aa transmembrane segment and a 108 aa cytoplasmic domain (13). Within the ECD, mouse IL­10 R beta shares 75% and 87% aa sequence identity with human and rat IL­10 R beta, respectively. Source: Recombinant protein corresponding to aa21-220 from IL-10 R beta Fc Chimera. Molecular Weight: ~70­80kD, Endotoxin: <0.10EU/1ug (LAL method). Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute in sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.