IL-4 (human), animal-free recombinant

Product Description: IL-4, a multifaceted cytokine, plays a crucial role in governing various T and B cell activities such as cell proliferation, survival, and gene expression. It is synthesized by mast cells, T cells, and bone marrow stromal cells. IL-4 specifically directs the transformation of naive CD4+ T cells into helper Th2 cells, known for their cytokine-secretion profile that includes IL-4, IL-5, IL-6, IL-10, and IL-13, all conducive to a humoral immune response. Additionally, IL-4 is pivotal in regulating the shift of immunoglobulin classes towards IgG1 and IgE isotypes. An important note is that excessive IL-4 production by Th2 cells can lead to heightened IgE production and allergic responses. The recombinant Human IL-4, a 15.1 kDa globular protein containing 130 amino acid residues, mirrors these regulatory functions in the immune system. . Amino acid sequence: MHKCDITLQE IIKTLNSLTE QKTLCTELTV TDIFAASKNT TEKETFCRAA TVLRQFYSHH EKDTRCLGAT AQQFHRHKQL IRFLKRLDRN LWGLAGLNSC PVKEANQSTL ENFLERLKTI MREKYSKCSS. Endotoxin concentration: <0.1 EU/µg. Activity: The ED50 was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is = 0.2 ng/ml, corresponding to a specific activity of > 5 x 10^6 units/mg. Contains no preservative. Protein function: Participates in at least several B-cell activation processes as well as of other cell types (PubMed:3016727). It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4. [The UniProt Consortium]