IL-37b, Recombinant, Human, aa46-218 (Interleukin 37 Isoform B, IL-1F7b)

IL-37b, Recombinant, Human, aa46-218 (Interleukin 37 Isoform B, IL-1F7b)
Item number Size Datasheet Manual SDS Delivery time Quantity Price
145805.25 25 µg - -

3 - 19 business days*

835.00€
 
Human Interleukin 1 family member 7 (IL-1F7), also named FIL-1 zeta, IL-37, IL-1H4, IL-1HL and... more
Product information "IL-37b, Recombinant, Human, aa46-218 (Interleukin 37 Isoform B, IL-1F7b)"
Human Interleukin 1 family member 7 (IL-1F7), also named FIL-1 zeta, IL-37, IL-1H4, IL-1HL and IL-1RP1, is an anti-inflammatory member of the IL-1 cytokine family. IL-1F7 shares ~21%, 24%, and 30% aa sequence identity with mature IL-1alpha, IL-1beta and IL-1ra, respectively. Mouse IL-1F7 has not been reported, but human IL-1F7 is active on mouse cells. Five isoforms (IL-1F7a through e) are generated through alternative exon usage and have distinct expression profiles (1-5). Like IL-1alpha, IL-1beta and IL-18, all of the IL-1F7 variants lack a typical signal peptide. The longest IL-1F7 transcript is the basis for the product offered here. This product, also referred to as IL-1F7b, or IL-1F7 isoform 1, is normally generated from a 218 amino acid (aa) proprotein that consists of a 45aa propeptide plus a 173 aa mature region that is cleaved by caspase-1 to generate the 32kD mature form (aa46-218) (6). IL-1F7 is up-regulated by inflammatory stimuli in peripheral blood mononuclear cells (7). Experimental overexpression of IL-1F7b in vivo limits the inflammatory response and protects mice from colitis and LPS-induced shock (7, 8). Both unprocessed and mature IL-1F7b can form homodimers in solution (6, 7). Although IL-1F7b will bind to IL-18 Ralpha with low affinity, this has no effect on IL-18 receptor activity (4, 6, 9). Alternatively, IL-1F7b will also bind to IL-18 BP, generating a complex that interacts with IL-18Rbeta. This has the effect of attenuating IL-18 activity via the IL-18 receptor (9). IL-1F7b can also function intracellularly. Following LPS-induced cleavage of its propeptide, IL-1F7b associates with Smad3 and translocates to the nucleus. This results in a reduction of pro-inflammatory cytokine secretion (10). Source: E. coli­derived recombinant protein corresponding to aa46-218 of human IL-37b/IL-1F7b, UniProt Accession #Q9NZH6, Molecular Weight: Predicted: 19.4kD, Observed: ~25kD (SDS-PAGE, reducing conditions), Endotoxin: <0.10EU/1ug (LAL method). Activity: Measured by its ability to enhance IL­18 BP inhibition of IL­18­induced IFNgamma release by KG-1 human acute myelogenous leukemia cells (Bufler, P. et al. (2002) PNAS 99:13723). The ED50 for this effect is typically 2.5­-12.5ug/ml. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplier: United States Biological
Supplier-Nr: 145805

Properties

Conjugate: No
Format: Highly Purified

Database Information

UniProt ID : Q9NZH6 | Matching products

Handling & Safety

Storage: -20°C
Shipping: +4°C (International: °C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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