IL-21, Recombinant, Human, aa30-162

IL-21, also known as Interleukin-21, is a T-helper cytokine that regulates humoral immunity and cell-mediated anti-tumor responses. It has broad immunoregulatory activity and can modulate both humoral and , cell-mediated responses. It is important in the regulation of haematopoiesis and immune responses, and can influence lymphocyte development. It is produced by activated T cell, and it influences proliferation of T and B cells and cytolytic activity of natural killer cells. It may be a critical factor in the control of persistent viral infections. It has a potential predicating significance for survival time in patients in the development of sporadic CRC. It binds to the IL-21R and can activate janus kinase JAK1, JAK3, STAT1, and STAT3 in EDgamma-16 cells. Source: Recombinant protein corresponding to aa30-162 from human IL-21, expressed in E. coli. Molecular Weight: ~15.5kD (134 aa), confirmed by MALDI-TOF, Endotoxin: ~1EU/1ug (LAL), Biological Activity: The activity is determined by the IFN-g ELISA in a using NK-92 human natural killer cells. The ED50 range < 4ng/ml. AA Sequence: MQGQDRHMIR MRQLIDIVDQ LKNYVNDLVP EFLPAPEDVE TNCEWSAFSC FQKAQLKSAN TGNNERIINV SIKKLKRKPP STNAGRRQKH RLTCPSCDSY EKKPPKEFLE RFKSLLQKMI HQHLSSRTHG SEDS, Applications: Suitable for use in Bioactivity and SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.