Hepatocyte growth factor (Hgf), partial, rat, recombinant

Organism: Rattus norvegicus (Rat). Source: E.coli. Expression Region: 477-728aa. Protein Length: Partial. Tag Info: N-terminal GST-tagged. Target Protein Sequence: TIVNLDHPVI SCAKTKQLRV VNGIPTQTTV GWMVSLKYRN KHICGGSLIK ESWVLTARQC FPARNKDLKD YEAWLGIHDV HERGEEKRKQ ILNISQLVYG PEGSDLVLLK LARPAILDNF VSTIDLPSYG CTIPEKTTCS IYGWGYTGLI NADGLLRVAH LYIMGNEKCS QHHQGKVTLN ESELCAGAEK IGSGPCEGDY GGPLICEQHK MRMVLGVIVP GRGCAIPNRP GIFVRVAYYA KWIHKVILTY KL. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Potent mitogen for mature parenchymal hepatocyte cells, ses to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization . Reference: Deduced primary structure of rat hepatocyte growth factor and expression of the mRNA in rat tissues.Toshiro K., Hagiya M., Nishizawa T., Seki T., Shimonishi M., Shimizu S., Nakamura T.Proc. Natl. Acad. Sci. U.S.A. 87:3200-3204(1990). Function: Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization (By similarity).