GDNF (human), animal-free recombinant

Product Description: GDNF is a homodimeric neurotrophic factor connected by disulfide bonds, sharing structural similarities with Artemin, Neurturin, and Persephin. These proteins are part of the cysteine-knot superfamily of growth factors, known for their stable dimeric structures. GDNF transmits signals through a complex receptor system involving RET and one of four GFRalpha receptors (alpha1-alpha4). Its specific actions include enhancing dopamine uptake and promoting the survival and morphological development of midbrain neurons. In a mouse model of Parkinson's disease, GDNF has demonstrated efficacy in improving symptoms such as bradykinesia, rigidity, and postural instability. The functional form of Human GDNF is a disulfide-linked homodimer composed of two 15 kDa polypeptide chains referred to as monomers. Each monomer contains seven conserved cysteine residues, including Cys-101, essential for inter-chain disulfide bridging, and others that contribute to the cysteine-knot configuration, forming an intramolecular ring. The molecular weight of Recombinant Human GDNF is calculated to be 30.4 kDa. . Amino acid sequence: MSPDKQMAVL PRRERNRQAA AANPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCDAAETTY DKILKNLSRN RRLVSDKVGQ ACCRPIAFDD DLSFLDDNLV YHILRKHSAK RCGCI. Endotoxin concentration: <0.1 EU/µg. Activity: Determined by a cell proliferation assay using SH-SY5Y cells. The expected ED50 for this effect is 1.0-10.0 ng/ml. Contains no preservative. Protein function: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high- affinity dopamine uptake. [The UniProt Consortium]