G-CSF, rHuman, Animal Free, High Purity/Bioactivity

Granulocyte Colony-Stimulating Factor (G-CSF , filgrastim, pegfilgrastim or lenograstim) is a hematopoietic growth factor that stimulates the proliferation and differentiation of hematopoietic progenitor cells committed to the neutrophil/granulocyte lineage, in a dose-dependent manner. It is produced by a variety of cells including macrophages, monocytes, fibroblasts, endothelial cells, and bone marrow stroma in response to specific stimulation by f.ex. endotoxin, TNF-alpha and IFN-gamma. Clinical applications of G-CSF include treatment of leukopenia following cancer therapy and bone marrow transplantation. Human and murine G-CSF are cross-species reactive. Source: Produced in the endosperm tissue of barley grain (Hordeum vulgare). Formulation: PBS pH 7.2, sterile filtered through 0.2 µm filter. Purity: Greater than 95% by SDS-PAGE gel analysis. Reconstitution: Note: Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile buffered saline to a concentration of no less than 100 µg/ml. For long term storage of the reconstituted solution it is recommended to add a carrier protein (0.1% HSA or BSA). Stability: The lyophilized protein, though stable at room temperature for few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles. Biological Activity The bioactivity of ISOkine(R) recombinant human G-CSF was assayed by measuring its dose dependent effect on proliferation of M-NFS-60 cells. The ED50 value for this ISOkine(R) is < 0.1 ng/ml corresponding to specific activity > 1 x 10e7 U/mg. We recommend that the optimal concentration for each specific application be determined by an initial dose-response assay. Each batch of ISOkine growth factor is tested for bioactivity and verified to have comparable activity to a commercial source. Endotoxin Level: Endotoxin level is less than 0.005ng per µg of ISOkine(R) product (0.05EU/µg). MAT Assay: Purified ISOkine(R) product carries no pyrogenic or pro-inflammatory contaminants, as assayed with monocyte activation test using Human 10-plex Cytokine Assay measuring IL-6, TNF-alpha and IL-1beta induction. ** Ref. The Blood Bank, University Hospital of Iceland, Reykjavik, Iceland Molecular Weight: Recombinant human G-CSF contains a 18.7 kDa protein with 174 amino acids and a 16 a.a. Histidine-based tag for a total length of 190a.a. and has a predicted molecular mass of 20.8 kDa.