Flt3-Ligand (human), animal-free recombinant

Product Description: Flt3-Ligand is a growth factor responsible for regulating the proliferation of early hematopoietic cells. It binds to cells expressing the Flt3 tyrosine kinase receptor. Interestingly, Flt3-Ligand itself doesn't directly stimulate the proliferation of early hematopoietic cells. Instead, it works synergistically with other colony-stimulating factors (CSFs) and interleukins to induce growth and differentiation. Unlike SCF, Flt3-Ligand doesn't affect mast cells. Various isoforms of Flt3-Ligand have been identified, with the predominant biologically active form being anchored to the cell surface as part of a transmembrane protein (209 amino acids). This membrane-bound isoform can undergo proteolytic cleavage, producing a biologically active soluble isoform. Recombinant Human Flt3-Ligand is a soluble protein weighing 17.6 kDa and composed of 155 amino acid residues.. Amino acid sequence: TQDCSFQHSP ISSDFAVKIR ELSDYLLQDY PVTVASNLQD EELCGGLWRL VLAQRWMERL KTVAGSKMQG LLERVNTEIH FVTKCAFQPP PSCLRFVQTN ISRLLQETSE QLVALKPWIT RQNFSRCLEL QCQPDSSTLP PPWSPRPLEA TAPTA. Endotoxin concentration: <0.1 EU/µg. Activity: The ED50 as determined by the dose-dependent stimulation of the proliferation of human AML5 cells is = 1.0 ng/ml, corresponding to a specific activity of > 1 x 10^6 units/mg. Contains no preservative. Protein function: Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins. [The UniProt Consortium]