Fibroblast Growth Factor 21, active (FGF21), Recombinant, Human

Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB. Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5ug/ml, corresponding to a specific activity of ? 2.0x10e3 IU/mg in the presence of 5ug/ml of rMuKlotho-beta and 10ug/ml of heparin. Sequence: HPIPDSSPLL QFGGQVRQRY LYTDDAQQTE AHLEIREDGT VGGAADQSPE SLLQLKALKP GVIQILGVKT SRFLCQRPDG ALYGSLHFDP EACSFRELLL EDGYNVYQSE AHGLPLHLPG NKSPHRDPAP RGPARFLPLP GLPPALPEPP GILAPQPPDV GSSDPLSMVG PSQGRSPSYA S, Molecular Weight: 19.4kD, PubMed ID: 10858549, 12975309, 15489334, 15340161, 15902306, 17623664, 18829467, 19117008, 20094046, Gene Name: FGF21, UNQ3115/PRO10196, Swiss Prot: Q9NSA1, Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.