Fibroblast growth factor 14 (FGF14), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 1-252aa. Protein Length: Full Length of Isoform 2. Tag Info: N-terminal GST-tagged. Target Protein Sequence: MVKPVPLFRR TDFKLLLCNH KDLFFLRVSK LLDCFSPKSM WFLWNIFSKG THMLQCLCGK SLKKNKNPTD PQLKGIVTRL YCRQGYYLQM HPDGALDGTK DDSTNSTLFN LIPVGLRVVA IQGVKTGLYI AMNGEGYLYP SELFTPECKF KESVFENYYV IYSSMLYRQQ ESGRAWFLGL NKEGQAMKGN RVKKTKPAAH FLPKPLEVAM YREPSLHDVG ETVPKPGVTP SKSTSASAIM NGGKPVNKSK TT. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Probably involved in nervous system development and function. Reference: "Fibroblast growth factor (FGF) homologous factors: new members of the FGF family implicated in nervous system development." Smallwood P.M., Munoz-Sanjuan I., Tong P., Macke J.P., Hendry S.H., Gilbert D.J., Copeland N.G., Jenkins N.A., Nathans J. Proc. Natl. Acad. Sci. U.S.A. 93:9850-9857(1996). Function: Probably involved in nervous system development and function.