Fibrinogen-like protein 1 (Fgl1), mouse, recombinant

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 23-314aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: LESESCLREQ VRLRAQVHQL ETRVKQQQTM IAQLLHEKEV QFLDKGSENS FIDLGGKRQY ADCSEIYNDG FKQSGFYKIK PLQSLAEFSV YCDMSDGGGW TVIQRRSDGS ENFNRGWNDY ENGFGNFVQN NGEYWLGNKN INLLTIQGDY TLKIDLTDFE KNSSFAQYQS FKVGDKKSFY ELNIGEYSGT AGDSLSGTFH PEVQWWASHQ RMKFSTWDRD NDNYQGNCAE EEQSGWWFNR CHSANLNGVY YRGSYRAETD NGVVWYTWHG WWYSLKSVVM KIRPSDFIPN II. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Immune suppressive molecule that inhibits antigen-specific T-cell activation by acting as a major ligand of LAG3. Responsible for LAG3 T-cell inhibitory function. Binds LAG3 independently from MHC class II (MHC-II). Secreted by, and promotes growth of, hepatocytes. Reference: "Fibrinogen-like protein 1 is a major immune inhibitory ligand of LAG-3." Wang J., Sanmamed M.F., Datar I., Su T.T., Ji L., Sun J., Chen L., Chen Y., Zhu G., Yin W., Zheng L., Zhou T., Badri T., Yao S., Zhu S., Boto A., Sznol M., Melero I. Chen L. Cell 0:0-0(2018). Function: nan