FGFb / FGF2 rHuman, Animal Free, High Purity/Bioactivity

FGF-basic (bFGF or FGF2) is a member of the fibroblast growth factor (FGF) family. FGF family members are heparin-binding multifunctional proteins and are involved in a variety of biological processes including angiogenesis, wound healing, and embryonic development. FGF-basic is known to play a key role during prenatal development and postnatal growth and regeneration of tissues by promoting cellular proliferation and differentiation. FGF basic has been recognized as a critical component of human embryonic stem cell culture medium due to its vital role for cells to remain in an undifferentiated state. FGF basic signals through FGFR 1b, 1c, 2c, 3c and 4. This recombinant human FGF-basic consists of 146 amino acid residues (FGFb146). In addition it contains a 10 aa tag at the N terminus resulting in a 17.8 kDa protein. Source: Produced in the endosperm tissue of barley grain (Hordeum vulgare). Formulation: PBS pH 7.2, sterile filtered Purity: Greater than 95% by SDS-PAGE gel analysis. Reconstitution: Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile phosphate buffered saline to a concentration of no less than 100 µg/ml. For long term storage of the reconstituted solution it is recommended to add a carrier protein (0.1% HSA or BSA). Stability: The lyophilized protein, though stable at room temperature for few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze thaw cycles. Biological Activity: The bioactivity of ISOkine(R) recombinant human FGF-basic 146 is assayed by measuring its dose dependent effect on proliferation of 3T3 cells. The ED50 for this effect using FGFb146 is < 0.4 ng/ml, corresponding to a specific activity > 2.5 x10e6 U/mg. We recommend that the optimal concentration for each specific application be determined by an initial dose-response assay. Each batch of ISOkine(R) growth factor is tested for bioactivity and verified to have comparable activity to a commercial source. Endotoxin Level: Endotoxin level is less than 0.005ng per µg of ISOkine(R) product (0.05EU/µg). MAT Assay: Purified ISOkine(R) product carries no pyrogenic or pro-inflammatory contaminants, as assayed with monocyte activation test using Human 10-plex Cytokine Assay measuring IL-6, TNF alpha and IL-1beta induction. ** Ref. The Blood Bank, University Hospital of Iceland, Reykjavik, Iceland Molecular Weight: This recombinant human FGF-basic consists of 146 amino acid residues. In addition it contains a 10 aa tag at the N-terminus resulting in a 17.8 kDa protein.