FGF-acidic (human), animal-free recombinant

Product Description: FGF-acidic belongs to the extensive FGF family, comprising 23 known members. These proteins are vital in prenatal development, postnatal growth, and tissue regeneration by encouraging cellular proliferation and differentiation. FGF-acidic, specifically, is a non-glycosylated growth factor that binds to heparin, found in various tissues such as the brain, kidney, retina, smooth muscle cells, bone matrix, osteoblasts, astrocytes, and endothelial cells. It interacts with all FGF receptors, showcasing its broad signaling capabilities. The recombinant Human FGF-acidic is a 16.8 kDa protein made up of 141 amino acid residues.. Amino acid sequence: MFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D. Endotoxin concentration: <0.1 EU/µg. Activity: Determined by a cell proliferation assay using Balb/c 3T3 cells. The expected ED50 is = 0.5 ng/ml in the presence of 10 µg/ml heparin, corresponding to a specific activity of > 2 x 106 units/mg. Contains no preservative. Protein function: Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Acts as a ligand for FGFR1 and integrins. Binds to FGFR1 in the presence of heparin leading to FGFR1 dimerization and activation via sequential autophosphorylation on tyrosine residues which act as docking sites for interacting proteins, leading to the activation of several signaling cascades. Binds to integrin ITGAV:ITGB3. Its binding to integrin, subsequent ternary complex formation with integrin and FGFR1, and the recruitment of PTPN11 to the complex are essential for FGF1 signaling. Induces the phosphorylation and activation of FGFR1, FRS2, MAPK3/ERK1, MAPK1/ERK2 and AKT1 (PubMed:18441324, PubMed:20422052). Can induce angiogenesis (PubMed:23469107). [The UniProt Consortium]