Draxin (DRAXIN), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 26-349aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: GALAPGTPAR NLPENHIDLP GPALWTPQAS HHRRRGPGKK EWGPGLPSQA QDGAVVTATR QASRLPEAEG LLPEQSPAGL LQDKDLLLGL ALPYPEKENR PPGWERTRKR SREHKRRRDR LRLHQGRALV RGPSSLMKKA ELSEAQVLDA AMEESSTSLA PTMFFLTTFE AAPATEESLI LPVTSLRPQQ AQPRSDGEVM PTLDMALFDW TDYEDLKPDG WPSAKKKEKH RGKLSSDGNE TSPAEGEPCD HHQDCLPGTC CDLREHLCTP HNRGLNNKCF DDCMCVEGLR CYAKFHRNRR VTRRKGRCVE PETANGDQGS FINV. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Chemorepulsive axon guidance protein required for the development of spinal cord and forebrain commissures. Acts as a chemorepulsive guidance protein for commissural axons during development. Able to inhibit or repel neurite outgrowth from dorsal spinal cord. Inhibits the stabilization of cytosolic beta-catenin (CTNNB1) via its interaction with LRP6, thereby acting as an antagonist of Wnt signaling pathway. Reference: "Neucrin is a novel neural-specific secreted antagonist to canonical Wnt signaling."Miyake A., Takahashi Y., Miwa H., Shimada A., Konishi M., Itoh N.Biochem. Biophys. Res. Commun. 390:1051-1055(2009). Function: Chemorepulsive axon guidance protein required for the development of spinal cord and forebrain commissures. Acts as a chemorepulsive guidance protein for commissural axons during development. Able to inhibit or repel neurite outgrowth from dorsal spinal cord. Inhibits the stabilization of cytosolic beta-catenin (CTNNB1) via its interaction with LRP6, thereby acting as an antagonist of Wnt signaling pathway.