Chordin-like protein 1 (Chrdl1), mouse, recombinant

Organism: Mus musculus (Mouse). Source: Baculovirus. Expression Region: 23-447aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: EQVKHSDTYC VFQDKKYRVG EKWHPYLEPY GLVYCVNCIC SENGNVLCSR VRCPSLHCLS PVHIPHLCCP RCPDSLPPVN NKVTSKSCEY NGTTYQHGEL FIAEGLFQNR QPNQCSQCSC SEGNVYCGLK TCPKLTCAFP VSVPDSCCRV CRGDAELSWE HADGDIFRQP ANREARHSYL RSPYDPPPNR QAGGLPRFPG SRSHRGAVID SQQASGTIVQ IVINNKHKHG QVCVSNGKTY SHGESWHPNL RAFGIVECVL CTCNVTKQEC KKIHCPNRYP CKYPQKIDGK CCKVCPEEPP SQNFDSKGSF CGEETMPVYE SVFMEDGETT RKVALETERP PQVEVHVWTI QKGILQHFHI EKISKRMFGE LHHFKLVTRT TLNQWKLFTE GEAQLSQMCS SQVCRTELED LVQVLYLGRP EKDHC. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Seems to antagonize the function of BMP4 by binding to it and preventing its interaction with receptors. Alters the fate commitment of neural stem cells from gliogenesis to neurogenesis. Contributes to neuronal differentiation of neural stem cells in the brain by preventing the adoption of a glial fate. May play a crucial role in dorsoventral axis formation. Antagonizes the function of BMP7 and may thus play an important role in the embryonic bone formation. Shows no inhibitory effect on the inducing activity of BMP2. Plays a role during anterior segment eye development. Reference: "Ventroptin: a BMP-4 antagonist expressed in a double-gradient pattern in the retina." Sakuta H., Suzuki R., Takahashi H., Kato A., Shintani T., Iemura S., Yamamoto T.S., Ueno N., Noda M. Science 293:111-115(2001). Function: Seems to antagonize the function of BMP4 by binding to it and preventing its interaction with receptors. Alters the fate commitment of neural stem cells from gliogenesis to neurogenesis. Contributes to neuronal differentiation of neural stem cells in the brain by preventing the adoption of a glial fate. May play a crucial role in dorsoventral axis formation (By similarity). Antagonizes the function of BMP7 and may thus play an important role in the embryonic bone formation. Shows no inhibitory effect on the inducing activity of BMP2. Plays a role during anterior segment eye development (By similarity).