Cerebral Dopamine Neurotrophic Factor, rat recombinant (rrCDNF)

CDNF rat recombinant produced in E. coli is a single, non-glycosylated polypeptide chain containing 163 amino acids and having a molecular mass of 18.8 kDa. CDNF is a member of the ARMET family and acts as a trophic factor for dopamine neurons. CDNF inhibits the 6-hydroxydopamine (6-OHDA)-induced degeneration of dopaminergic neurons. When CDNF controlled after 6-OHDA-lesioning, it reestablishes the dopaminergic function and inhibits the degeneration of dopaminergic neurons in substantia nigra. CDNF is universally expressed in neuronal and non-neuronal tissues. The highest levels in the brain are found in the optic nerve and corpus callosum. CDNF rat is able to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons when immobilized at 5 - 25 µg/mL on a nitrocellulose-coated microplate.