C-C motif chemokine 4, active (CCL4), Recombinant, Human

Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5-mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B. {ECO:0000269, PubMed:10540332, ECO:0000269, PubMed:12070155, ECO:0000269, PubMed:8525373}. Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human CCR5 transfected murine BaF3 cells is less than 2.0ng/ml, corresponding to a specific activity of ? 5.0x10e5 IU/mg. Sequence: APMGSDPPTA CCFSYTARKL PRNFVVDYYE TSSLCSQPAV VFQTKRGKQV CADPSESWVQ EYVYDLELN, Molecular Weight: 7.8kD, PubMed ID: 2462251, 2521882, 2521353, 2247088, 2568930, 1894635, 15843566, 16625196, 15489334, 2809212, 9521068, 8525373, 10540332, 12070155, 12401480, 8134838, Gene Name: CCL4,LAG1,MIP1B,SCYA4, Swiss Prot: P13236, Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.