Bone morphogenetic protein 15 (BMP15), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 268-392aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: QADGISAEVT ASSSKHSGPE NNQCSLHPFQ ISFRQLGWDH WIIAPPFYTP NYCKGTCLRV LRDGLNSPNH AIIQNLINQL VDQSVPRPSC VPYKYVPISV LMIEANGSIL YKEYEGMIAE SCTCR. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: May be involved in follicular development. Oocyte-specific growth/differentiation factor that stimulates folliculogenesis and granulosa cell (GC) growth. Reference: "Characterization of the post-translational modification of recombinant human BMP-15 mature protein." Saito S., Yano K., Sharma S., McMahon H.E., Shimasaki S. Protein Sci. 17:362-370(2008). Function: nan