LysoBrite(TM) NIR

LysoBrite(TM) NIR
Item number Size Datasheet Manual SDS Delivery time Quantity Price
ABD-22641 500 tests -

3 - 8 business days*

171.00€
 
Ex: 636 nm. Em: 650 nm. Lysosomes are cellular organelles which contain acid hydrolase enzymes to... more
Product information "LysoBrite(TM) NIR"
Ex: 636 nm. Em: 650 nm. Lysosomes are cellular organelles which contain acid hydrolase enzymes to break up waste materials and cellular debris. Lysosomes digest excess or worn-out organelles, food particles, and engulfed viruses or bacteria. The membrane around a lysosome allows the digestive enzymes to work at pH 4.5. The interior of the lysosomes is acidic (pH 4.5-4.8) compared to the slightly alkaline cytosol (pH 7.2). The lysosome maintains this pH differential by pumping protons from the cytosol across the membrane via proton pumps and chloride ion channels. LysoBriteT NIR selectively accumulates in lysosomes probably via the lysosome pH gradient. The lysotropic indicator is a hydrophobic compound that easily permeates intact live cells, and trapped in lysosomes after it gets into cells. Its fluorescence is significantly enhanced upon entering lysosomes. This key feature significantly reduces its staining background and makes it useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. It is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells. LysoBriteT dyes significantly outperform the equivalent LysoTracker Tdyes (from Invitrogen). LysoBriteT dyes can stay in live cells for more than a week with very minimal cell toxicity while the LysoTracker dyes can only be used for a few hours.
Supplier: AAT Bioquest
Supplier-Nr: 22641

Properties

Application: Cell-based assays, Fluorescent lysosome labeling

Database Information

Handling & Safety

Storage: -20°C
Shipping: +20°C (International: +20°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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