CLF [Cholyl-Lys-Fluorescein]

CLF is a fluorescein-labeled bile acid analog that closely parallels the cellular binding and uptake properties of cholic acid derivatives. It is a divalent 'unipolar' anionic fluorescent monohydroxy bile salt analog with physical, biological and cholestatic properties that are similar to those of lithocholate, glycolithocholate and their derivatives and thus offers a potentially useful probe for studying mechanisms of monohydroxy bile salt-induced cholestasis at the hepatocellular level. CLF is currently used as a tool for the visualization of bile acid transport within liver tissue. Solvent: DMSO. Spectral properties: Absorbance (nm): 487, Correction Factor (260 nm): 0.32, Correction Factor (280 nm): 0.35, Extinction coefficient (cm^-1 M^-1): 80000 (0.1N NaOH), Excitation (nm): 498, Emission (nm): 517, Quantum yield: 0.7900 (EtOH (neutral)), 0.95 (0.1N NaOH).