CFSE Fluorescent Cellular Stain (5,6 CFDA,SE)

Non-fluorescent and moderately hydrophobic in its di-esterified form, 5,6 CFDA,SE easily penetrates lipid bi-layers of living cells and is quickly converted to the amine-reactive, green fluorescent form (CFSE) upon cleavage of the acetate groups by intracellular esterases. This highly fluorescent form is membrane-impermeant and is retained on and within the cells, while any excess CFSE probe is easily washed away in subsequent wash steps. Excitation: 492 nm. Emission: 520-540 nm.