CFSE [5-(and 6)-Carboxyfluorescein diacetate, succinimidyl ester] *Mixed isomers*

CFSE [5-(and 6)-Carboxyfluorescein diacetate, succinimidyl ester] *Mixed isomers*
Item number Size Datasheet Manual SDS Delivery time Quantity Price
ABD-22028 2500 µg (5 x 500µg) -

3 - 8 business days*

112.00€
 
It is widely recognized that fluorescent labeling of cells is an effective means to determine... more
Product information "CFSE [5-(and 6)-Carboxyfluorescein diacetate, succinimidyl ester] *Mixed isomers*"
It is widely recognized that fluorescent labeling of cells is an effective means to determine total cell numbers or how many viable cells exist in a sample. Flow cytometry combined with fluorescent staining is a powerful tool to analyze heterogeneous cell populations. Fluorescein diacetate (FDA) and its derivatives are non-fluorescent molecules that diffuse into cells and are hydrolyzed by intracellular non-specific esterases to give fluorescent products. The fluorescent products can be accumulated only in those cells that have intact cell membranes, therefore, dead cells with leaky membranes are not stained. The precise kinetics of membrane transport and intracellular hydrolysis of FDA and its analogs (such as CDCFDA) are related to cellular functions, thus FDA labeling can be used for monitoring cells by flow cytometry or fluorescence microscopy. The fluorescence intensity of labeled cells by FDA dyes varies considerably among cell lines and strains, probably because of differences in intracellular esterase activity. CFDA, SE is an amine-reactive FDA derivative that can be used to prepare a variety of FDA conjugates. AAT Bioquest offers this convenient package size for flow cytometric use. Each vial of CFSE may be reconstituted to a stock concentration of 10 mM with 90 uL of anhydrous DMSO. The DMSO stock solution should be protected from light and stored at -20°C with dessicant, avoiding freeze-thawing.
Supplier: AAT Bioquest
Supplier-Nr: 22028

Properties

Application: FC

Database Information

CAS : 150347-59-4| Find alternatives

Handling & Safety

Storage: -20°C
Shipping: +20°C (International: +20°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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