CFDA-SE

CFDA-SE is a stable, cell-permeable dye that consists of a fluorescein molecule containing two acetate moieties and a succinimidyl ester (SE) functional group. Upon diffusion into the intracellular environment, the acetate groups are cleaved by cellular esterases leaving CFSE (Cay-16802), which is fluorescent and not cell permeable. CFSE covalently couples to intracellular molecules via its succinimidyl group and can be retained in cells for at least eight weeks. CFDA-SE is often used to assay cell proliferation as it is partitioned with high fidelity between daughter cells for up to eight generational divisions. CFSE, the cleavage product of CFDA-SE, displays excitation/emission maxima of 491/518 nm, respectively.Formal Name: 3',6'-bis(acetyloxy)-3-oxo-2,5-dioxo-1-pyrrolidinyl ester-spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-ar-carboxylic acid. CAS Number: 150347-59-4. Synonyms: 5(6)-Carboxyfluorescein diacetate succinimidyl ester, 5(6)-CFDA N-succinmidyl ester. Molecular Formula: C29H19NO11. Formula Weight: 557.5. Purity: >95% (mixture of regioisomers). Emission: 518 nm. Excitation: 491 nm. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 30 mg/ml, DMF:PBS (pH 7.2)(1:3): 0.25 mg/ml, DMSO: 20 mg/ml. SMILES: CC(OC1=CC2=C(C3(C4=C(C=C(OC(C)=O)C=C4)O2)C5=C(C(O3)=O)C=C(C(ON6C(CCC6=O)=O)=O)C=C5)C=C1)=O.CC(OC7=CC8=C(C9(C%10=C(C(O9)=O)C=CC(C(ON%11C(CCC%11=O)=O)=O)=C%10)C%12=C(C=C(OC(C)=O)C=C%12)O8)C=C7)=O. InChi Code: InChI=1S/2C29H19NO11/c1-14(31)37-17-4-7-21-23(12-17)39-24-13-18(38-15(2)32)5-8-22(24)29(21)20-6-3-16(11-19(20)28(36)40-29)27(35)41-30-25(33)9-10-26(30)34,1-14(31)37-17-4-7-20-23(12-17)39-24-13-18(38-15(2)32)5-8-21(24)29(20)22-11-16(3-6-19(22)28(36)40. InChi Key: LZFDYQHOQDFMOA-UHFFFAOYSA-N.