HhAntag

HhAntag is an inhibitor of the Sonic Hedgehog (Shh) pathway (EC50 = 0.4 µM for Shh signaling in a reporter assay). It inhibits proliferation of GNP cells when used at concentrations ranging from 0.1 nM to 25 µM. In vivo, HhAntag (20 or 100 mg/kg) suppresses Gli1 mRNA levels, a target of Shh signaling, and increases tumor cell death in a mouse model of Ptc1+/-p53-/- medulloblastoma. HhAntag also blocks basal and bone morphogenic protein 2-induced chondrogenesis in micromass cultures of mouse limb mesenchymal cells.Formal Name: N-[4-chloro-3-[6-(dimethylamino)-1H-benzimidazol-2-yl]phenyl]-3,5-dimethoxy-benzamide. CAS Number: 496794-70-8. Molecular Formula: C24H23ClN4O3. Formula Weight: 450.9. Purity: >95%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 1 mg/mL, DMSO: 2 mg/mL, DMSO:PBS (pH 7.2) (1:2): 0.3mg/mL, Ethanol: 1 mg/mL. lambdamax: 213 nm. SMILES: CN(C)C1=CC=C2C(NC(C3=CC(NC(C4=CC(OC)=CC(OC)=C4)=O)=CC=C3Cl)=N2)=C1. InChi Code: InChI=1S/C24H23ClN4O3/c1-29(2)16-6-8-21-22(12-16)28-23(27-21)19-11-15(5-7-20(19)25)26-24(30)14-9-17(31-3)13-18(10-14)32-4/h5-13H,1-4H3,(H,26,30)(H,27,28). InChi Key: UBHJFPVTEATUFS-UHFFFAOYSA-N.