Description: HhAntag is a small molecule inhibitor of GLI1-mediated transcription, an essential down-stream element of the Hedgehog (Hh) pathway with the anti-tumor activity. Target: Smo, Hedgehog/Smoothened. Smiles: COc1cc(OC)cc(c1)C(=O)Nc1ccc(Cl)c(c1)-c1nc2cc(ccc2[nH]1)N(C)C. References: Actis, Marcelo, Connelly,...

HhAntag is an inhibitor of the Sonic Hedgehog (Shh) pathway (EC50 = 0.4 µM for Shh signaling in a reporter assay). It inhibits proliferation of GNP cells when used at concentrations ranging from 0.1 nM to 25 µM. In vivo, HhAntag (20 or 100 mg/kg) suppresses Gli1 mRNA levels, a target of Shh signaling, and increases...