7beta,25-dihydroxy Cholesterol

An active metabolite of cholesterol, activates EBI2/GPR183 in a GTPgammaS assay (EC50 = 221 nM) but does not bind to EBI2 in CHO cell membranes expressing the human receptor in a radioligand binding assay (IC50 = 2,632 nM), acts as a cellular chaperone that corrects the localization defect of NPC1I1061T in HEK293 cells expressing the mutant transporter (EC50 = 1.9 µM). Formulation: A solid. InChI: InChI=1S/C27H46O3/c1-17(7-6-12-25(2,3)30)20-8-9-21-24-22(11-14-27(20,21)5)26(4)13-10-19(28)15-18(26)16-23(24)29/h16-17,19-24,28-30H,6-15H2,1-5H3/t17-,19+,20-,21+,22+,23+,24+,26+,27-/m1/s1. InChIKey: BQMSKLCEWBSPPY-CGSQRZAOSA-N. SMILES: O[C@H](C1)CC[C@@]2(C)C1=C[C@H](O)[C@]3([H])[C@]2([H])CC[C@@]4(C)[C@@]3([H])CC[C@]4([H])[C@H](C)CCCC(C)(O)C