Lysates

A cell lysate is produced by enzymatic, osmotic or mechanical disruption of the plasma membrane of a population of cells. Cell lysis is utilized during the purification of cellular components such as DNA, RNA, proteins or whole organelles. A lysate of cells over-expressing a certain protein is commonly used as a positive control in western blots and ELISAs to evaluate the target specificity of an antibody.

A cell lysate is produced by enzymatic, osmotic or mechanical disruption of the plasma membrane of a population of cells. Cell lysis is utilized during the purification of cellular components such... read more »
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Lysates

A cell lysate is produced by enzymatic, osmotic or mechanical disruption of the plasma membrane of a population of cells. Cell lysis is utilized during the purification of cellular components such as DNA, RNA, proteins or whole organelles. A lysate of cells over-expressing a certain protein is commonly used as a positive control in western blots and ELISAs to evaluate the target specificity of an antibody.

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HEK293 Whole Cell Lysate Insulin Stimulated
HEK293 Whole Cell Lysate Insulin Stimulated

Item number: W09-001-A78

The cells were grown in Dulbecco's medium supplemented with 10% fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodium deoxycholic acid , 0.1% SDS and 0.01% (w/v) sodium azide to...
Application: WB
Host: Human
Species reactivity: human
287.00€ *
Review
HeLa Whole Cell Lysate - (Doxorubicin Stimulated  1.0 mg/ml by Lowry assay in 1X SDS-PAGE Sample Buf
HeLa Whole Cell Lysate - (Doxorubicin Stimulated 1.0...

Item number: W09-001-A79

Ready to use. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added).
Application: WB, Positive control
287.00€ *
Review
HeLa Whole Cell Lysate - (Nocodozole Stimulated  1.0 mg/ml by Lowry assay in 1X SDS-PAGE Sample Buff
HeLa Whole Cell Lysate - (Nocodozole Stimulated 1.0...

Item number: W09-001-A81

Ready to use. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added).
Application: WB, Positive control
287.00€ *
Review
HeLa Cell Nuclear Extract - Doxorubicin Stimulated (1.0 mg/ml by Lowry assay in 1X SDS-PAGE Sample B
HeLa Cell Nuclear Extract - Doxorubicin Stimulated (1.0...

Item number: W09-001-A82

Ready to use. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added).
Application: WB, Positive control
272.00€ *
Review
HeLa Cell Nuclear Extract - Etoposide Stimulated (1.0 mg/ml by Lowry assay in 1X SDS-PAGE Sample Buf
HeLa Cell Nuclear Extract - Etoposide Stimulated (1.0...

Item number: W09-001-A83

Ready to use. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added).
Application: WB, Positive control
272.00€ *
Review
MDA-MB-468 Whole Cell Lysate
MDA-MB-468 Whole Cell Lysate

Item number: W09-001-GG9

The cells were grown in Dulbecco's medium supplemented with 10% fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodium deoxycholic acid , 0.1% SDS and 0.01% (w/v) sodium azide to...
Application: WB
200.00€ *
Review
Normal Mouse Heart Whole Cell Lysate
Normal Mouse Heart Whole Cell Lysate

Item number: W10-000-T014

1.0 mg/ml by Lowry assay in 1X SDS-PAGE Sample Buffer
Application: WB
204.00€ *
Review
Normal Mouse Liver Whole Cell Lysate
Normal Mouse Liver Whole Cell Lysate

Item number: W10-000-T020

1.0 mg/ml by Lowry assay in 1X SDS-PAGE Sample Buffer
Application: WB
204.00€ *
Review
RAW 264.7 Whole Cell Lysate
RAW 264.7 Whole Cell Lysate

Item number: W10-001-369

1.0 mg/ml by Lowry assay in 1X SDS-PAGE Sample Buffer
Application: WB
204.00€ *
Review
Mouse Embryonic Fibroblast Whole Cell Lysate
Mouse Embryonic Fibroblast Whole Cell Lysate

Item number: W10-001-371

1.0 mg/ml by Lowry assay in 1X SDS-PAGE Sample Buffer
Application: WB
204.00€ *
Review
NIH/3T3 Whole Cell Lysate PDGF Stimulated
NIH/3T3 Whole Cell Lysate PDGF Stimulated

Item number: W10-001-377

The cells were grown in Dulbecco's medium supplemented with 10% fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodium deoxycholic acid , 0.1% SDS and 0.01% (w/v) sodium azide to...
Application: WB
Host: Mouse
Species reactivity: mouse
204.00€ *
Review
C2C12 Whole Cell Lysate
C2C12 Whole Cell Lysate

Item number: W10-001-GL7

C2C12 cells were grown in Dulbecco's medium supplemented with 10% fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodium deoxycholic acid , 0.1% SDS and 0.01% (w/v) sodium azide to...
Application: ChIP, IP, WB
Host: Mouse
Species reactivity: mouse
200.00€ *
Review
3 from 18 pages