Total MMP-13, Human, BioAssay(TM) DuoSet ELISA Development Kit

The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP). Sample Type: Cell culture supernates , Intended Use: This BioAssay(TM) DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human active and Pro-Matrix Metalloproteinase 13 (Total MMP-13). This DuoSet is designed for the analysis of cell culture supernates. Range: ~62.5-4000pg/ml, Specificity: Recognizes natural and recombinant human active and Pro-Matrix Metalloproteinase 13 (Total MMP-13). This DuoSet recognizes the pro form of recombinant human (rh) MMP-13 and the mature form of rhMMP-13 by itself or when complexed with rhTIMP-1. Kit Components: Mouse anti-human MMP-13 capture antibody, 1x vial, Biotinylated goat anti-human MMP-13 detection antibody, 1x vial, Recombinant human MMP-13 standard, 1x 3vials, Streptavidin-HRP, 1x vial, Storage and Stability: Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.