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Product information "Prostaglandin E2 ELISA Kit - Monoclonal"
Prostaglandin E2 (PGE2) is a primary product of arachidonic acid metabolism in many cells. Like most eicosanoids, it does not exist preformed in any cellular reservoir. When cells are activated or exogenous free arachidonate is supplied, PGE2 is synthesized de novo and released into the extracellular space. In vivo, PGE2 is rapidly converted to an inactive metabolite (13,14-dihydro-15-keto PGE2) by the prostaglandin 15-dehydrogenase pathway. The half-life of PGE2 in the circulatory system is approximately 30 seconds and normal plasma levels are 3-12 pg/ml. Our PGE2 ELISA has been validated for use with urine, plasma, and culture media samples. In general, urine and culture media samples can be diluted, if necessary, and added directly to the assay well. Plasma samples should be purified prior to use. Because of the rapid metabolism of PGE2, the determination of in vivo PGE2 biosynthesis is often best accomplished by the measurement of PGE2 metabolites. Our Prostaglandin E Metabolite assay (Cay-514531) converts all major PGE2 metabolites into a single stable derivative which is easily measurable by ELISA.Synonyms: PGE2 EIA Kit - Monoclonal. Assay Range: 7.8-1,000 pg/ml. Sensitivity: 80% B/B0: 15 pg/ml. Mid-point: 50% B/B0: 30-70 pg/ml. Cross Reactivity: (Prostaglandin E2) 100%, (Prostaglandin E2 Ethanolamide) 100%, (Prostaglandin E2-1-glyceryl ester) 100%, (Prostaglandin E3) 43.0%, (Prostaglandin E1) 18.7%, (8-iso Prostaglandin E2) 2.5%, (Sulprostone) 1.25%, (6-keto Prostaglandin F1alpha) 1.0%, (8-iso Prostaglandin F2alpha) 0.25%, (Prostaglandin A2) 0.04%, (13,14-dihydro-15-keto Prostaglandin E2) 0.02%, (Arachidonic Acid) <0.01%, (Arachidonoyl Ethanolamide) <0.01%, (O-Arachidonoyl Ethanolamide) <0.01%, (Butaprost) <0.01%, (Conjugated Linoleic Acid (10E,12Z)) <0.01%, (8(S),15(S)-DiHETE) <0.01%, (Leukotriene B4) <0.01%, (Misoprostol) <0.01%, (Misoprostol (free acid)) <0.01%, (tetranor-PGEM) <0.01%, (tetranor-PGFM) <0.01%, (Prostaglandin A1) <0.01%, (8-iso Prostaglandin A2) <0.01%, (Prostaglandin A3) <0.01%, (Prostaglandin B1) <0.01%, (Prostaglandin B2) <0.01%, (Prostaglandin D2) <0.01%, (11-deoxy Prostaglandin E2) <0.01%, (16,16-dimethyl Prostaglandin E2) <0.01%, (19(R)-hydroxy Prostaglandin E2) <0.01%, (20-hydroxy Prostaglandin E2) <0.01%, (15-keto Prostaglandin E2) <0.01%, (Prostaglandin F1alpha) <0.01%, (Prostaglandin F2alpha) <0.01%, (13,14-dihydro-15-keto Prostaglandin F2alpha) <0.01%, (Prostaglandin F3alpha) <0.01%, (Prostaglandin J2) <0.01%, (15-deoxy-Delta12,14-Prostaglandin J2) <0.01%, (Thromboxane B2) <0.01%. Origin: Animal/Goat, Animal/Mouse, Animal/Eel, Animal/Bovine.
This website uses cookies, which are necessary for the technical operation of the website and are always set. Other cookies, which increase the usability of this website, serve for direct advertising or simplify interaction with other websites and social networks, will only be used with your consent.
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