Fetuin, Human, BioAssay ELISA Kit (alpha-2 Heremans-Schmid Glycoprotein, FHSG, Fetuin A)

Human Fetuin-A ELISA is a sandwich enzyme immunoassay for the quantitative measurement of human Fetuin-A protein in serum. It is intended for in vitro research use only. Features:  The total assay time is less than 3 hours.,  The kit measures serum Fetuin-A/AHSG.,  Standard is human plasma based.,  Quality Controls are human serum based.,  Components in the kit are provided ready-to-use, concentrated and lyophilized. Kit Components: F4102-01A: Microtiter Strips, 1x96 wells,, F4102-01B: Pab (HRP), 1x13ml, *F4102-01C: Master Calibrator, 1x100ng, F4102-01D: High Control, 2x1vial, F4102-01E: Low Control, 2x1vial, F4102-01F: Dilution Buffer, 10x, 1x20ml, F4102-01G: Wash Solution, 10x, 1x100ml, F4102-01H: Substrate Solution (TMB), 1x13ml, F4102-01J: Stop Solution, H2SO4, 1x13ml, Sensitivity: Limit of Detection (LOD), defined as concentration of analyte giving absorbance higher than mean absorbance of blank* plus three standard deviations of the absorbance of the blank: Ablank + 3xSDblank, is calculated from real fetuin-A values in wells is 0.35ng/ml. *Dilution buffer is pipetted into blank wells. Specificity: Highly specific for human Fetuin-A protein. Crossreactivity was observed with monkey. No crossreactivity with serum of other animal species (bovine, feline, canine, hamster, goat, equine, mouse, porcine, rabbit, rat, sheep) has been observed. Storage and Stability: Store at 4°C. Stable for 6 months at 4°C. , *Note: Store F4102-01C at 4°C as a lyophilized powder. Upon reconstitution, aliquot and store at -20°C. Stable for 6 months at -20ºC.