Aquaporin 2, Collecting Duct (AQP2) BioAssay(TM) ELISA Kit (Human)

Aquaporin 2, Collecting Duct (AQP2) BioAssay(TM) ELISA Kit (Human)
Item number Size Datasheet Manual SDS Delivery time Quantity Price
023553.96 96 tests - -

4 - 22 business days

792.00€
 
Aquaporin 2, Collecting Duct (AQP2) BioAssay(TM) ELISA Kit is a sandwich enzyme immunoassay for... more
Product information "Aquaporin 2, Collecting Duct (AQP2) BioAssay(TM) ELISA Kit (Human)"
Aquaporin 2, Collecting Duct (AQP2) BioAssay(TM) ELISA Kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of AQP2 in human tissue homogenates, urine and other biological fluids. Detection Range: 0.312-20ng/ml, Sensitivity: 0.114ng/ml, Precision: Intra-Assay %CV: <10%, Inter-Assay %CV: <12%, Kit Components: *023553A: Microtiter Plate, 1x48 wells or 1x96 wells, Pre-coated, ready to use, *023553B: Standard, 2x1vial , 023553C: Standard Diluent, 1x10ml or 1x20ml, *023553D: Detection Reagent A (green), 1x60ul or 1x120ul , *023553E: Detection Reagent B (red), 1x60ul or 1x120ul , 023553F: Assay Diluent A, 1x6ml or 1x12ml, 023553G: Assay Diluent B, 1x6ml or 1x12ml, 023553H: TMB Substrate, 1x4.5ml or 1x9ml, *023553J: Positive Control, 4.95ng/ml, 1x1vial (Lyophilized), 023553K: Stop Solution, 1x3ml or 1x6ml, 023553L: Wash Buffer, 30X, 1x10ml or 1x20ml , Precaution: The Stop Solution (023553K) included for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Storage and Stability: Store *023553A, *023553B, *023553D, *023553E and *023553J at -20°C. After reconstitution of *023553J, store at 4°C and use within 5 days. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Notes on 023553J: Positive Control , Intended Use:, The positive control is from a human sample known to contain the AQP2. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly. Characteristics: The positive control vial contains human AQP2 (lyophilized powder). Note: The human based material used in this product is screened for HIV, Hepatitis A, B, C, HTLV and Syphilis. Expected Values: 4.95ng/ml, Usage: Dissolve with 150ul standard diluent and let stand for 10 minutes. If powder is partly insoluble, shake gently until fully dissolved. Mix gently before use. In the experiment, add 100ul to an appropriate well of the plate and test its concentration along with standards and your specimens. Note: Upon receipt store Positive Control vial at -20°C. After reconstitution, store Positive Control vial at 4°C and use within 5 days. Assay Procedure Summary: 1. Prepare all reagents, samples and standards., 2. Add 100ul standard or sample to each well. Incubate 1 hour at 37°C., 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C., 4. Aspirate and wash 3 times., 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C., 6. Aspirate and wash 5 times., 7. Add 90ul TMB Substrate. Incubate 10-20 minutes at 37°C., 8. Add 50ul Stop Solution. Read at 450nm immediately.
Keywords: AQP2, AQP-2, AQP-CD, WCH-CD, Aquaporin-2, Aquaporin-CD, ADH water channel, Collecting duct water channel protein, Water channel protein for renal collecting duct
Supplier: United States Biological
Supplier-Nr: 023553

Properties

Application: ELISA
Reactivity: Human
Format: Solid Phase

Handling & Safety

Storage: vT
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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