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Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
Cay10006910-96 | 96 wells | - |
6 - 10 business days* |
661.00€
|
If you have any questions, please use our Contact Form.
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
The HIF (hypoxia-inducible factor) transcription factor is a member of the basic-helix-loop-helix... more
Product information "HIF-1alpha Transcription Factor Assay Kit"
The HIF (hypoxia-inducible factor) transcription factor is a member of the basic-helix-loop-helix (bHLH) family of transcription factors and plays an important role in maintaining cellular oxygen homeostasis. HIF-1alpha has emerged as an important drug target in breast and prostate cancer, cardiovascular disease, and ischemia. Cayman's HIF-1alpha Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysate. A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) consensus sequence containing the HIF-1alpha response element is immobilized to the wells of a 96-well plate. HIF-1alpha contained in a nuclear extract or whole cell lysates, binds specifically to the HIF-1alpha response element. The HIF transcription factor complex is detected by addition of a specific primary antibody directed against HIF-1alpha. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm.Synonyms: ARNT-interacting Protein, Hypoxia-Inducible Factor-1alpha. Origin: Animal/Bovine, Animal/Rabbit, Animal/Goat.
Keywords: | Hypoxia-Inducible Factor-1alpha |
Supplier: | Cayman Chemical |
Supplier-Nr: | 10006910 |
Properties
Application: | HIF DNA binding |
Species reactivity: | human, mouse, rat |
Format: | Solid Phase |
Database Information
Handling & Safety
Storage: | -80°C |
Shipping: | -80°C (International: -80°C) |
Signal Word: | Danger |
GHS Hazard Pictograms: |
|
H Phrases: | H360 |
P Phrases: | P201, P202, P280, P308+P313, P405, P501 |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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