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Product information "Purification Media, Affinity, Protein A Beads"
The origin of Protein A is from the bacteria Staphylococcus aureus. Protein A is capable of binding most classes and subclasses of immunoglobulins from goat, rabbit, mouse and human., Protein A beads are produced using genetically engineered form of Protein A. Nonessential regions have been removed while leaving the immunoglobulins binding sites intact. The recombinant Protein A has been immobilized to agrose beads using TargetLock chemistry, in order to create a more stable product for the affinity purification and immunoprecipitation of various mammalian immunoglobulins. Protein A beads are being routinely used for the isolation of IgG from several species of mammals. The binding of Protein A to IgGs vary between animal species and between IgG subclasses within the same species. Major limitation of protein A lies with the weak binding it presents towards mouse IgG1, a common IgG subclass. Despite that, Protein A possesses useful, properties that make it a popular choice for the isolation of most types of IgGs. Protein A binds IgGs through the Fc region of the molecules leaving the Fab region available for binding the antigen. Protein A Beads are commomnly used for antibodies purification and for the isolation of immune complexes by Immunoprecipitation. Protein A Beads Specifications: , Matrix: , Sepharose CL-4B, Coupling method: TargetLock. Protein A Density: 2-4mg/ml of resin , Binding Capacity: , ~4-8mg rabbit IgG per ml of resin, Mean bead size: 40 -165um, Bead structure: Highly cross-linked spherical agarose, 4%, Max back pressure: 0.3 MPa, 3 bar, Max. flow rate: 4 ml/min/cm2, Recommended flow rate: 1-3 ml/min/cm2, Stability of the matrix: pH 2-11, Storage and Stability: May be stored at 4°C. Do not freeze. Stable for at least 6 months.
This website uses cookies, which are necessary for the technical operation of the website and are always set. Other cookies, which increase the usability of this website, serve for direct advertising or simplify interaction with other websites and social networks, will only be used with your consent.
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