Anti-Prostaglandin D Synthase (hematopoietic-type, human) (Clone 2A5)

Prostaglandin D synthase (PGDS) is a glutathione-dependent enzyme and member of the sigma class of glutathione-S-transferases (GSTs) that catalyzes the conversion of PGH2 (Cay-17020) to PGD2 (Cay-12010), an eicosanoid that has numerous biological functions, including vasorelaxation, recruitment of inflammatory cells, and inhibition of platelet aggregation. There are two types of PGDS: lipocalin PGDS (L-PGDS, Cay-10006788, Cay-10006787) and hematopoietic PGDS (H-PGDS, Cay-10006593). H-PGDS is found in peripheral tissues and immune cells, including Th2 cells, antigen-presenting cells, mast cells, megakaryocytes, and eosinophils, where it is localized to the cytosol. H-PGDS activity is increased by a variety of stimuli, including LPS, anti-IgE antibodies, phorbol 12-myristate 13-acetate (TPA, Cay-10008014), ionomycin (Cay-10004974), and inflammatory cytokines such as IL-13, IL-3, or IL-4. siRNA silencing of Hpgds decreases LPS-induced production of PGD2 in mouse bone marrow-derived macrophages (BMDMs). Transgenic overexpression of HPGDS in mice increases croton oil-induced ear swelling and PGD2 production, and genome-wide deletion of Hpgds exacerbates hypotension and vascular permeability in a mouse model of anaphylaxis. H-PGDS protein levels are increased in the nasal mucosa of patients with allergic rhinitis, and HPGDS SNPs have been found in individuals with asthma. Cayman's Prostaglandin D Synthase (hematopoietic-type, human) Monoclonal Antibody (Clone 2A5) can be used for immunohistochemistry (IHC) and Western blot (WB) applications.Synonyms: Hematopoietic-PGDS, H-PGDS, H-PGD Synthase, PGD Synthase (hematopoietic-type). Immunogen: Recombinant human H-PGDS. Clone Designation: 2A5. Formulation: (Request formulation change), 100 µg purified monoclonal antibody. Storage Buffer: PBS, pH 7.2, with 50% glycerol, 0.5 mg/ml BSA, and 0.02% sodium azide. Host: Mouse. Isotype: IgG2bkappa. Applications: IHC, WB. Cross Reactivity: (+) H-PGDS. Origin: Animal/Mouse.