Conjugation Kits

AAT Bioquest's iFluor(TM) dyes are developed for labeling proteins, in particular, antibodies. These dyes are optimized to have minimal fluorescence quenching effect on proteins and nucleic acids. iFluor(TM) 700 dyes have fluorescence excitation and emission maxima close to 690 nm and 710 nm respectively. These...

AAT Bioquest's iFluor(TM) dyes are developed for labeling proteins, in particular, antibodies. These dyes are optimized to have minimal fluorescence quenching effect on proteins and nucleic acids. iFluor(TM) 790 dyes have fluorescence excitation and emission maxima close to 790 nm and 810 nm respectively. These...

Ex: 616 nm. Em: 647 nm. Soluble in: Water. C-Phycocyanin (C-PC) occurs as the major phycobiliprotein in many cyanobacteria and as a secondary phycobiliprotein in some red algae. The pigment has a single visible absorption maximum between 615 and 620 nm and a fluorescence emission maximum at ~650 nm. Its molecular...

Oregon Green(R) is a trademark of Life Technologies.

Ex: 565 nm. Em: 575 nm. Soluble in: DMSO. R-Phycoerythrin (PE) is an orange fluorescent protein which has an excitation wavelength of 565 nm and an emission wavelength of 575 nm. AAT Bioquest offers this BuccutiteT rapid labeling kit to facilitate the PE conjugations to antibodies and other proteins such as...

Protein-protein conjugations are commonly performed with a bifunctional linker such as SMCC. One end of the SMCC reacts (via NHS ester) with amines (-NH2) found in the amino acid lysine and N-terminus, and the other end reacts (via maleimide) with the thiol groups (-SH) found in the amino acid cysteine. However,...

The E. coli in vivo biotinylation kit comes in a convenient, easy-to-use format and contains all the components necessary to label biotin-acceptor peptides with biotin in E. coli. Strain BL21, a chemically competent E. coli B strain. The cells include an IPTG-inducible plasmid containing the BirA gene and the...

Mix-n-Stain(TM) Small Ligand Labeling Kits are designed for rapid labeling of low molecular weight (MW 150 -5000) ligands without a purification step. Suitable ligands or substrates include SNAP-tag(R), CLIP-tag(TM) and HaloTag(R) ligand derivatives that have an aliphatic amine. CF(R) dyes are Biotium's line of...

AAT Bioquest's iFluor(TM) dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555 and 633 nm). iFluor(TM) 350 dyes have...

Ex: 657 nm. Em: 700 nm. Soluble in: DMSO. AAT Bioquest's mFluorT dyes are developed for flow cytometry-focused applications. These dyes have large Stokes Shifts, and can be well excited by the laser lines of flow cytometers (e.g., 405 nm, 488 nm and 633 nm). mFluorT Red 700 dyes have fluorescence excitation and...

Ex: 629 nm. Em: 780 nm. Soluble in: DMSO. AAT Bioquest's mFluorT dyes are developed for flow cytometry-focused applications. These dyes have large Stokes Shifts, and can be well excited by the laser lines of flow cytometers (e.g., 405 nm, 488 nm and 633 nm). mFluorT Red 780 dyes have fluorescence excitation and...

AAT Bioquest's iFluor(TM) dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555 and 633 nm). iFluor(TM) 350 dyes have...