E. coli in vivo Biotinylation Kit

The E. coli in vivo biotinylation kit comes in a convenient, easy-to-use format and contains all the components necessary to label biotin-acceptor peptides with biotin in E. coli. Strain BL21, a chemically competent E. coli B strain. The cells include an IPTG-inducible plasmid containing the BirA gene and the resistance gene for streptomycin or spectinomycin. Just transform the competent cells with a vector expressing your protein of interest with an AviTag(TM) sequence (example: pAN or pAC vectors). After transformation, BirA expression is induced and biotin is added to the culture. The BirA efficiently catalyzes the specific attachment of biotin to the AviTag.