Cookie preferences
This website uses cookies, which are necessary for the technical operation of the website and are always set. Other cookies, which increase the comfort when using this website, are used for direct advertising or to facilitate interaction with other websites and social networks, are only set with your consent.
Configuration
Technically required
These cookies are necessary for the basic functions of the shop.
"Allow all cookies" cookie
"Decline all cookies" cookie
CSRF token
Cookie preferences
Currency change
Customer-specific caching
FACT-Finder tracking
Individual prices
Selected shop
Session
Comfort functions
These cookies are used to make the shopping experience even more appealing, for example for the recognition of the visitor.
Note
Show the facebook fanpage in the right blod sidebar
Statistics & Tracking
Affiliate program
Conversion and usertracking via Google Tag Manager
Track device being used
Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
U1000-86N.100 | 100 µg | - | - |
3 - 19 business days* |
834.00€
|
If you have any questions, please use our Contact Form.
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
Source:|Recombinant corresponding to aa2-end of human UbcH5a with N-terminal HIS tag expressed in... more
Product information "Ubiquitin Conjugating Enzyme E2 D1, N-terminal His tag, Recombinant, Human (E2(17)KB1, SFT, Stimulat"
Source:, Recombinant corresponding to aa2-end of human UbcH5a with N-terminal HIS tag expressed in E. coli (NM_003338). Specific Activity: 50ul reaction mix (4mM ATP, 5mM MgCl2, 1mM DTT, UBE1 (0.5ug), UbcH5a or other E2, Biotin-Ubiquitin (0.5ug) and Smurf2 (2) in 50mM Tris pH 7.4 for 30 min at 37°C, then add to streptavidin coated plates for 30 min at 37°C. Incubate with anti-Smurf2, then Europium-labeled secondary antibody, and detect by time resolved fluorescence (lambda-ex=330nm, lambda-em=620nm). Applications: Suitable for use in the study of enzyme kinetics, screening inhibitors and selectivity profiling. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing. Store at -70°C. Aliquots are stable for at least 6 months at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Keywords: | SFT, UbcH5, UBE2D1, EC=6.3.2.19, UBC4/5 homolog, Stimulator of Fe transport, Ubiquitin-protein ligase D1, Ubiquitin carrier protein D1, Ubiquitin-conjugating enzyme E2 D1, Ubiquitin-conjugating enzyme E2(17)KB 1, Ubiquitin-conjugating enzyme E2-17 kDa 1 |
Supplier: | United States Biological |
Supplier-Nr: | U1000-86N |
Properties
Conjugate: | No |
Species reactivity: | human |
Format: | Purified |
Database Information
KEGG ID : | K06689 | Matching products |
UniProt ID : | P51668 | Matching products |
Gene ID | GeneID 7321 | Matching products |
Handling & Safety
Storage: | -80°C |
Shipping: | -20°C (International: -20°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
more
You will get a certificate here
Viewed