Transmembrane prolyl 4-hydroxylase (P4HTM), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 82-563aa. Protein Length: Partial of Isoform 3. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: VHYSNGDESS DPGPQHRAQG PGPEPTLGPL TRLEGIKVGH ERKVQLVTDR DHFIRTLSLK PLLFEIPGFL TDEECRLIIH LAQMKGLQRS QILPTEEYEE AMSTMQVSQL DLFRLLDQNR DGHLQLREVL AQTRLGNGWW MTPESIQEMY AAIKADPDGD GVLSLQEFSN MDLRDFHKYM RSHKAESSEL VRNSHHTWLY QGEGAHHIMR AIRQRVLRLT RLSPEIVELS EPLQVVRYGE GGHYHAHVDS GPVYPETICS HTKLVANESV PFETSCRQVS PNWGLPSILR PGTPMTQAQP CTVGVPLGMG PGDHWVIPVS PWEHPQLGTC SVPPLPYSYM TVLFYLNNVT GGGETVFPVA DNRTYDEMSL IQDDVDLRDT RRHCDKGNLR VKPQQGTAVF WYNYLPDGQG WVGDVDDYSL HGGCLVTRGT KWIANNWINV DPSRARQALF QQEMARLARE GGTDSQPEWA LDRAYRDARV EL. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF1A at 'Pro-402' and 'Pro-564'. May function as a cellular oxygen sensor and, under normoxic conditions, may target HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Reference: An endoplasmic reticulum transmembrane prolyl 4-hydroxylase is induced by hypoxia and acts on hypoxia-inducible factor alpha.Koivunen P., Tiainen P., Hyvaerinen J., Williams K.E., Sormunen R., Klaus S.J., Kivirikko K.I., Myllyharju J.J. Biol. Chem. 282:30544-30552(2007). Function: Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF1A at 'Pro-402' and 'Pro-564'. May function as a cellular oxygen sensor and, under normoxic conditions, may target HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex.