T4 beta-glucosyltransferase, C-terminal His-tag

T4-phage beta-glucosyltransferase, also known as UDP glucose-DNA beta-glucosyltransferase, Genbank Accession No. NP_049658, a.a. 1-351(end) with C-terminal His-tag, MW= 41.6 kDa, expressed in E. coli. Useful for the differentiation of hydroxymethylcytosine (HMC) from methylcytosine in DNA, via glucosylating HMC and protecting HMC from endonuclease cleavage. Formulated in 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, 20% glycerol. Stability >6 months at -80°C. References: 1. Song, C.X., et al. (2011). Nat Biotechnol. 29(1): 68-72. 2. Kornberg, S.R., et al. (1961). J. Biol. Chem. 236: 1487-1493.