Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 (ATP2A1), partial, human, recombinant

Organism: Homo sapiens (Human). Source: in vitro E.coli expression system. Expression Region: 10-450aa. Protein Length: Partial. Tag Info: N-terminal 10xHis-tagged. Target Protein Sequence: EECLAYFGVS ETTGLTPDQV KRNLEKYGLN ELPAEEGKTL WELVIEQFED LLVRILLLAA CISFVLAWFE EGEETITAFV EPFVILLILI ANAIVGVWQE RNAENAIEAL KEYEPEMGKV YRADRKSVQR IKARDIVPGD IVEVAVGDKV PADIRILAIK STTLRVDQSI LTGESVSVIK HTEPVPDPRA VNQDKKNMLF SGTNIAAGKA LGIVATTGVG TEIGKIRDQM AATEQDKTPL QQKLDEFGEQ LSKVISLICV AVWLINIGHF NDPVHGGSWF RGAIYYFKIA VALAVAAIPE GLPAVITTCL ALGTRRMAKK NAIVRSLPSV ETLGCTSVIC SDKTGTLTTN QMSVCKMFII DKVDGDICLL NEFSITGSTY APEGEVLKND KPVRPGQYDG LVELATICAL CNDSSLDFNE AKGVYEKVGE ATETALTTLV E. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: n/a. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Key regulator of striated muscle performance by acting as the major Ca(2+) ATPase responsible for the reuptake of cytosolic Ca(2+) into the sarcoplasmic reticulum. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction. Reference: n/a. Function: nan